Research Assistant/Tech 3 – Lange

Job title:

Research Assistant/Tech 3 – Lange

Company

University of British Columbia

Job description

Staff – Non UnionJob Category Non Union Technicians and Research AssistantsJob Profile Non Union Salaried – Research Assistant /Technician 3Job Title Research Assistant/Tech 3 – LangeDepartment Lange Laboratory | Department of Pathology and Laboratory Medicine | Faculty of MedicineCompensation Range $4,567.04 – $5,385.67 CAD MonthlyPosting End Date December 15, 2024Note: Applications will be accepted until 11:59 PM on the Posting End Date.Job End Date Jan 1, 2026The anticipated start date for this position is January 2, 2025. The term is for one year with the possibility of extension.In your application please include (1) a cover letter, and (2) a CV or resume.At UBC, we believe that attracting and sustaining a diverse workforce is key to the successful pursuit of excellence in research, innovation, and learning for all faculty, staff and students. Our commitment to employment equity helps achieve inclusion and fairness, brings rich diversity to UBC as a workplace, and creates the necessary conditions for a rewarding career.Job SummaryThe Lange laboratory for translational proteomics at The University of British Columbia and BC Children’s Hospital investigates innovative approaches to precision medicine for childhood cancer patients. We strive to develop new diagnostic and therapeutic approaches to detect and treat children suffering from cancer earlier, better and with reduced impact on their life. To reach this goal, we use mass spectrometry-based proteomics; biochemistry; cell biology; and molecular biology approaches to study protein function and modification in childhood cancers and develop precision medicine pipelines. We are highly collaborative at the local, national and international levels. Our work has and continues to advance understanding of protein modification, improve proteomic investigation on small patient biopsies and changed patient care.We are seeking an exceptional research technician (mass spectrometry / laboratory management) to pursue this goal together with us and join our dynamic translational research team. Your main role will be maintaining the group’s mass spectrometers and contributing to the team’s research activities by performing proteomics sample preparation and assisting with method development. You will also be responsible for laboratory management and will conduct experiments designed to improve technology and investigate protein function and modification in cultured cells, animal models and patient biopsies. We are seeking an experienced individual with strong skills and expertise in proteomics, mass spectrometry and biochemistry as well as autonomy in design, execution and statistical analysis of experiments.We Offer:A dynamic and diverse work environment.An attractive compensation package including health benefitsThe opportunity to take the lead in building a new mass spectrometry facility at the BC Children’s Hospital Research Institute.The opportunity to work with and learn from world experts in proteomics at UBC, BC Cancer Research Centre and the BCCHRI.The opportunity to conduct research and develop new methods.Plentiful opportunities to advance your skills and take on new roles.Organizational Status
The successful candidate will report to the principal investigator.Work Performed

• Independently maintain, calibrate, and operate liquid chromatography tandem mass spectrometry instrumentation
• Routine preparation of proteomics samples (tissue lysis, protein precipitation, labeling, digestion, SCX, HILIC, SEC chromatography, …)
• LC-MS/MS analysis of complex proteomes from various sources.
• Contribute to and perform biological experiments related to research in a range of normal and cancer tissues/cells including leukemia and medulloblastoma; troubleshoot problems as they arise
• Contributes to the development of new analytical techniques and methodologies
• Act as the main personnel in communicating with collaborators; perform proof-of-concept experiments and technique training; advise on experiment setup and data analysis
• Independently perform a variety of cell biological and biochemical techniques including western blotting, protein expression/purification/quantification, cell culture.
• Assist with overseeing the operation of the laboratory including inventory management
• Assist with technique and safety training for students and junior lab staff
• Contribute to the preparation of research publications and grants
• Participate in meetings related to technical and scientific aspects of the projects in the laboratory.
• Other related tasks and duties as required.

Consequence of Error/Judgement
The successful applicant is expected to exercise a considerable amount of judgment, responsibility, and initiative in determining work procedures and methods. He/she should carefully observe and evaluate their work. The principal investigator will meet regularly with the individual to discuss methodology, results and problems. Consistent poor judgment decisions or errors can result in incorrect data interpretation and potential loss of funding.Supervision Received
Works under minimum supervision, receiving specific instructions only on unusual problems or when receiving new projects outlines from the principal investigator.Supervision Given
Assist with training students and junior lab staff, provide input on staff/co-op student selection and performance evaluation.Minimum Qualifications
Completion of a relevant technical program or a university degree in a relevant discipline and a minimum three years of related experience or an equivalent combination of education and experience.– Willingness to respect diverse perspectives, including perspectives in conflict with one’s own– Demonstrates a commitment to enhancing one’s own awareness, knowledge, and skills related to equity, diversity, and inclusionPreferred Qualifications

• Graduate degree preferred, in molecular or cellular biology, microbiology, genetics, chemistry or a related discipline.
• Demonstrated ability to work independently and communicate effectively verbally and in writing.
• Knowledge and experience in operation and maintenance of nano-LC systems for protein and peptide separation.
• Knowledge and experience in operation, calibration and maintenance of mass spectrometers for proteomics.
• Knowledge and experience in the evaluation of MS spectra and raw data at the instrument level. Experience in down-stream data analysis including database searching and protein quantitation an asset.
• Proven troubleshooting skills in LC-MS/MS instrumentation and workflows.
• Knowledge and experience in proteomics sample preparation techniques for complex proteomes (lysis, precipitation, reduction, alkylation, digestion, de-salting, …)
• Experience in proteomics techniques (shot gun, dimethyl labeling, phospho-enrichment, single cell) and other laboratory techniques (i.e., SDS page, western blot, cell culture, immunohistology, …) is an asset.
• Experience in laboratory management is an asset.
• Strong organizational skills and ability to work in a team-oriented environment.
• All relevant UBC safety courses including biosafety and chemical safety are required but can be completed during orientation.

Expected salary

$4567.04 – 5385.67 per month

Location

Canada

Job date

Wed, 20 Nov 2024 08:43:58 GMT

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